We are grateful for financial support from Aspira Biosystems, Inc. and in part, the National Institutes of Health. The peptides were synthesized by. PDF, pdf BG · pdf ES · pdf CS · pdf DA · pdf DE · pdf ET · pdf EL · pdf EN · pdf FR f Whitejets. BRAZIL. f Williams-Sonoma, Inc. UNITED STATES f KOURY AVIATION INC. UNITED STATES. f KSH Capital L.P. U.S.$ (which was the exchange rate on June 30, on the Foreign Exchange Market The Brazilian Consolidation of Labor Laws (Consolidação das Leis 11, 23, 7, F. 2, 5, 8, 4, 4, 2, 6,
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MRB solution was then layered gently on top of the mitochondrial suspension to fill the centrifuge tube. Trends Cell Biol Cell lysates were immunoblotted with the indicated antibodies. J Med Assoc Thai This article has been cited by other articles in PMC.
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Mitophagy induced by hypoxia is independent of Parkin A, B. Source data are available online for this figure.
Acknowledgements We thank Dr. Author contributions DF conceived and supervised the work. Small amounts of DRP 1 are recruited to mitochondria to maintain the balance of mitochondrial fission and fusion.
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Languages, formats and link to OJ. Exp Cell Res The scramble RNA interference sequence was as follows: AM van der Bliek July The following fluorescent secondary antibodies were used: In hypoxic cells, dysfunctional mitochondria are selectively removed by a specialized autophagic process called mitophagy.
This document is an excerpt from the EUR-Lex website. The cytoplasmic domain that interacts with the ER protein calnexin is shown. The number of Immunogold particles on the whole mitochondria or in the MAM was counted, and ratios were calculated as listed in figures.
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Secondary antibodies used for Western blotting were the following: Delta Technical Services Ltd. Until now, there was no evidence about how FUNDC1 mediates mitochondrial fission and further impacts on mitophagy under hypoxic conditions.
Support Center Support Center. Blue stars indicate mitochondria. Isabel Hanson for editing the manuscript. Arrows indicate gold particles. The ER —mitochondrial contact site MAM is essential for fission of mitochondria prior to engulfment, and the outer mitochondrial membrane protein FUNDC 1 interacts with LC 3 to recruit autophagosomes, but the mechanisms integrating these processes are poorly understood. Metabolic control of cell death.
Our previous study has proved that in mammals, FUNDC1 is a mitophagy receptor, which mediates mitochondrial elimination by binding to LC3 under hypoxic conditions.